![34446801 eutron 34446801 eutron](https://i1.wp.com/grizzlybearsims.com/wp-content/uploads/2021/01/FlightSimulator_rY9FVNAjlq.jpg)
Engraftment levels of individual mice (n = 3 per group) are shown.
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Once engraftment reached > 0.1%, mice were treated IP with Dex-NV (dashed coloured line), free Dex (solid coloured line) both at 2.5 mg/kg or placebo (grey line) 5 times per week over 28 days. 1–3, respectively, were inoculated into NSG mice and the levels of human cells (CD45 + and CD7 +) were measured in weekly PB aspirates. Data represent mean ± SD of at least 2 replicate measurements. Viability was assessed by flow cytometry using Annexin-V and PI, expressed as a proportion of the untreated control sample. 1–6) and normal bone marrow (NBM, n = 3) treated with Dex-NV (dashed lines) or free Dex (solid lines) for 48 h. The study describes a non-toxic, more easily scalable system for improving Dex solubility for use in cancer and can be applied to other medical conditions associated with inflammation.Įfficacy of Dex-NV in vitro and in vivo. NV accumulated in major organs, including bone marrow and brain, known sanctuary sites for ALL. Broad distribution of NV was seen rapidly following inoculation into mice. Importantly, high levels of DMSO solvent were not required in the NV formulations. Dex-NV were just as effective as the free drug against primary human leukemia cells in vitro and in vivo. Non-toxic, fully biodegradable Dex loaded nanovectors (NV) were formulated, via simple direct hydration within 10 min, as a vehicle to extend exposure and distribution in vivo. Data from clinical trials indicates that the pharmacokinetics of Dex vary considerably between patients and prolonging drug exposure rather than increasing absolute dose may improve efficacy. Mariko Tamaki is currently attending York University working a master's degree in women's studies.Dexamethasone (Dex) is a highly insoluble front-line drug used in cancer therapy. She has appeared widely on radio and television including First Person Singular on CBC radio and Imprint on TVO. Mariko Tamaki has performed her work across Canada and through the States, recently appearing at the Calgary Folkfest 2004, Vancouver Writer's Festival 2003, Spatial III, and the Perpetual Motion/Girls Bite Back Tour, which circled though Ottawa, Montreal, Brooklyn and Chicago. Mariko's third book, FAKE ID, is due out in spring 2005. Her well-received novel, Cover Me (McGilligan Books) was followed by a short fiction collection, True Lies: The Book of Bad Advice (Women's Press). B, was staged at the 2004 Rhubarb Festival at Buddies in Bad Times Theatre. She works and performs with fat activists Pretty Porky and Pissed Off and the theatre troupe TOA, whose recent play, A vs. Her well-received novel, Cover Me (McGilligan Books) was followed by a short fiction collection, True Lies: The Book of Bad Advice Mariko Tamaki is a Toronto writer, playwright, activist and performer. Mariko Tamaki is a Toronto writer, playwright, activist and performer.